The maximum concentration of substrate above which increase of substrate concentration does not increase the rate of reaction is called _______ of enzymes with substrate.

B. Saturation

Enzymes are biological catalysts that accelerate the rate of chemical reactions. As the concentration of substrate increases, the rate of the reaction also increases due to more frequent collisions between enzyme and substrate molecules. However, there’s a limit:

• Saturation: At a certain point, all the active sites of the enzyme molecules become occupied by substrate molecules. Increasing the substrate concentration further won’t affect the reaction rate because there are no free enzyme molecules available to bind with the additional substrate. This maximum concentration of substrate is called the saturation concentration.

The other options don’t accurately describe this concept:

• Termination: Termination refers to the final step in a reaction pathway, not the limitation of substrate binding.
• Fluctuation: Fluctuation implies random variations, not the specific point of maximum binding.
• Maximum Optimation: This term isn’t commonly used in enzyme kinetics.